| First Author | Bianchi LM | Year | 2002 |
| Journal | J Histochem Cytochem | Volume | 50 |
| Issue | 12 | Pages | 1641-5 |
| PubMed ID | 12486086 | Mgi Jnum | J:123993 |
| Mgi Id | MGI:3720307 | Doi | 10.1177/002215540205001208 |
| Citation | Bianchi LM, et al. (2002) Lac z Histochemistry and immunohistochemistry reveal ephrin-B ligand expression in the inner ear. J Histochem Cytochem 50(12):1641-5 |
| abstractText | Immunostaining in transgenic mice carrying the lac z gene can be used to map gene and protein distribution in a single tissue. In this study, we examined inner ears from ephrin-B3 homozygous and ephrin-B2 heterozygous mice. Ephrin-B3 lac z expression was limited in these mice. However, immunostaining revealed ephrin-B3 throughout cochlear and vestibular regions. Immunoreactivity was absent in ephrin-B3-homozygous null mutants, demonstrating the specificity of the antibody. Ephrin-B2 lac z reactivity was detected in a limited number of cells in cochlear and vestibular regions. Different immunostaining patterns were found with different antibodies. Comparison with lac z expression indicated which antibody was specific for the transmembrane-bound ephrin-B2 ligand. |
