| First Author | Słuczanowska-Głąbowska S | Year | 2013 |
| Journal | J Ovarian Res | Volume | 6 |
| Issue | 1 | Pages | 67 |
| PubMed ID | 24063422 | Mgi Jnum | J:310305 |
| Mgi Id | MGI:6761729 | Doi | 10.1186/1757-2215-6-67 |
| Citation | Sluczanowska-Glabowska S, et al. (2013) The effect of calorie restriction on the presence of apoptotic ovarian cells in normal wild type mice and low-plasma-IGF-1 Laron dwarf mice. J Ovarian Res 6(1):67 |
| abstractText | BACKGROUND: It is known that caloric restriction extends lifespan and can minimize age-related dysfunction of the reproductive system. We became interested in how caloric restriction influences apoptosis, which is a crucial process that maintains ovarian cell homeostasis. METHODS: We examined ovarian cells in: 2.5-year-old wild type mice on caloric restriction (CR) or fed ad libitum (AL) and Laron dwarf mice (GHR-KO) at the same ages on CR or fed AL. Apoptosis was assessed by histochemical analysis on paraffin sections of ovarian tissue. RESULTS: Morphological and histochemical analysis revealed that CR improved reproductive potential in 2.5-year-old WT littermates and GHR-KO female mice, as indicated by the increased number of ovarian follicles. The level of apoptosis in ovarian tissue was higher in WT mice on a CR diet compared with WT mice on the AL diet. In GHR-KO mice, the level of apoptosis in ovaries was similar for mice on CR and on AL diets and bigger than in WT mice on CR. CONCLUSIONS: Morphological and histochemical analysis revealed a younger biological age of the ovaries in 2-year-old WT littermates and GHR-KO female mice on CR compared with animals fed AL. |
