| Primary Identifier | MGI:2388703 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Trp53 |
| Transmission | Germline | Strain of Origin | 129S/SvEv-Gpi1<c> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The gene was disrupted using a two-step targeting strategy. First, exons 2-10 were replaced with a neo-TK cassette via homologous recombination. Next, correctly targeted ES cells were subjected to another round of gene targeting to replace the neo-TK cassette with a lacZ cassette. Successful gene replacement was selected for via gancyclovir resistance. Homozygous mutant animals were successfully established. |
