| First Author | Yan X | Year | 2022 |
| Journal | Redox Biol | Volume | 56 |
| Pages | 102457 | PubMed ID | 36063729 |
| Mgi Jnum | J:344250 | Mgi Id | MGI:7341017 |
| Doi | 10.1016/j.redox.2022.102457 | Citation | Yan X, et al. (2022) Role and mechanism of REG2 depletion in insulin secretion augmented by glutathione peroxidase-1 overproduction. Redox Biol 56:102457 |
| abstractText | We previously reported a depletion of murine regenerating islet-derived protein 2 (REG2) in pancreatic islets of glutathione peroxidase-1 (Gpx1) overexpressing (OE) mice. The present study was to explore if and how the REG2 depletion contributed to an augmented glucose stimulated insulin secretion (GSIS) in OE islets. After we verified a consistent depletion (90%, p < 0.05) of REG2 mRNA, transcript, and protein in OE islets compared with wild-type (WT) controls, we treated cultured and perifused OE islets (70 islets/sample) with REG2 (1 mug/ml or ml . min) and observed 30-40% (p < 0.05) inhibitions of GSIS by REG2. Subsequently, we obtained evidences of co-immunoprecipitation, cell surface ligand binding, and co-immunofluorescence for a ligand-receptor binding between REG2 and transmembrane, L-type voltage-dependent Ca(2+) channel (CaV1.2) in beta TC3 cells. Mutating the C-type lectin binding domain of REG2 or deglycosylating CaV1.2 removed the inhibition of REG2 on GSIS and(or) the putative binding between the two proteins. Treating cultured OE and perifused WT islets with REG2 (1 mug/ml or ml . min) decreased (p < 0.05) Ca(2+) influx triggered by glucose or KCl. An intraperitoneal (ip) injection of REG2 (2 mug/g) to OE mice (6-month old, n = 10) decreased their plasma insulin concentration (46%, p < 0.05) and elevated their plasma glucose concentration (25%, p < 0.05) over a 60 min period after glucose challenge (ip, 1 g/kg). In conclusion, our study identifies REG2 as a novel regulator of Ca(2+) influx and insulin secretion, and reveals a new cascade of GPX1/REG2/CaV1.2 to explain how REG2 depletion in OE islets could decrease its binding to CaV1.2, resulting in uninhibited Ca(2+) influx and augmented GSIS. These findings create new links to bridge redox biology, tissue regeneration, and insulin secretion. |
