| First Author | Kamachi F | Year | 2014 |
| Journal | Biochem Biophys Res Commun | Volume | 444 |
| Issue | 2 | Pages | 235-40 |
| PubMed ID | 24462862 | Mgi Jnum | J:218141 |
| Mgi Id | MGI:5616713 | Doi | 10.1016/j.bbrc.2014.01.060 |
| Citation | Kamachi F, et al. (2014) OX40 ligand regulates splenic CD8(-) dendritic cell-induced Th2 responses in vivo. Biochem Biophys Res Commun 444(2):235-40 |
| abstractText | In mice, splenic conventional dendritic cells (cDCs) can be separated, based on their expression of CD8alpha into CD8(-) and CD8(+) cDCs. Although previous experiments demonstrated that injection of antigen (Ag)-pulsed CD8(-) cDCs into mice induced CD4 T cell differentiation toward Th2 cells, the mechanism involved is unclear. In the current study, we investigated whether OX40 ligand (OX40L) on CD8(-) cDCs contributes to the induction of Th2 responses by Ag-pulsed CD8(-) cDCs in vivo, because OX40-OX40L interactions may play a preferential role in Th2 cell development. When unseparated Ag-pulsed OX40L-deficient cDCs were injected into syngeneic BALB/c mice, Th2 cytokine (IL-4, IL-5, and IL-10) production in lymph node cells was significantly reduced. Splenic cDCs were separated to CD8(-) and CD8(+) cDCs. OX40L expression was not observed on freshly isolated CD8(-) cDCs, but was induced by anti-CD40 mAb stimulation for 24 h. Administration of neutralizing anti-OX40L mAb significantly inhibited IL-4, IL-5, and IL-10 production induced by Ag-pulsed CD8(-) cDC injection. Moreover, administration of anti-OX40L mAb with Ag-pulsed CD8(-) cDCs during a secondary response also significantly inhibited Th2 cytokine production. Thus, OX40L on CD8(-) cDCs physiologically contributes to the development of Th2 cells and secondary Th2 responses induced by Ag-pulsed CD8(-) cDCs in vivo. |
