| First Author | Besouw MT | Year | 2012 |
| Journal | Fertil Steril | Volume | 98 |
| Issue | 1 | Pages | 162-5 |
| PubMed ID | 22578532 | Mgi Jnum | J:333294 |
| Mgi Id | MGI:6840267 | Doi | 10.1016/j.fertnstert.2012.03.050 |
| Citation | Besouw MT, et al. (2012) Studying nonobstructive azoospermia in cystinosis: histologic examination of testes and epididymis and sperm analysis in a Ctns(-)/(-) mouse model. Fertil Steril 98(1):162-5 |
| abstractText | OBJECTIVE: To study the pathogenesis of male infertility in cystinosis due to nonobstructive azoospermia, using a Ctns(-/-) mouse model. DESIGN: Observational case-control study. SETTING: Academic research laboratory. ANIMAL(S): Male C57BL/6 Ctns(-/-) mice were compared with C57BL/6 wild-type (wt) mice. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Fertility was studied using litter size (n = 3 vs. n = 2). After animals were sacrificed, testes, epididymis, and vas deferens were removed for testicular cystine measurements (n = 5 vs. n = 6), histologic studies (n = 3 vs. n = 3), and sperm analysis (n = 3 vs. n = 3). RESULT(S): Mean testicular cystine content was significantly higher in Ctns(-/-) mice compared with wt mice (26.6 +/- 1.22 vs. 0.1 +/- 0.01 nmol cystine/mg protein). Testes of Ctns(-/-) mice had lower weight compared with wt mice (0.096 +/- 0.009 g vs. 0.112 +/- 0.004 g), but mice fertility was similar (litter size 6.6 +/- 1.4 vs. 6.3 +/- 2.6 pups). Neither histologic nor sperm abnormalities were found. CONCLUSION(S): The Ctns(-/-) mouse model generated on C57BL/6 background is not suitable for clarifying the pathogenesis of male infertility in cystinosis. The etiology of nonobstructive azoospermia in these patients remains unclear. |
