| First Author | O'Neill KR | Year | 2012 |
| Journal | J Orthop Res | Volume | 30 |
| Issue | 8 | Pages | 1271-6 |
| PubMed ID | 22247070 | Mgi Jnum | J:355129 |
| Mgi Id | MGI:7737860 | Doi | 10.1002/jor.22071 |
| Citation | O'Neill KR, et al. (2012) Fracture healing in protease-activated receptor-2 deficient mice. J Orthop Res 30(8):1271-6 |
| abstractText | Protease-activated receptor-2 (PAR-2) provides an important link between extracellular proteases and the cellular initiation of inflammatory responses. The effect of PAR-2 on fracture healing is unknown. This study investigates the in vivo effect of PAR-2 deletion on fracture healing by assessing differences between wild-type (PAR-2(+/+)) and knock-out (PAR-2(-/-)) mice. Unilateral mid-shaft femur fractures were created in 34 PAR-2(+/+) and 28 PAR-2(-/-) mice after intramedullary fixation. Histologic assessments were made at 1, 2, and 4 weeks post-fracture (wpf), and radiographic (plain radiographs, micro-computed tomography (microCT)) and biomechanical (torsion testing) assessments were made at 7 and 10 wpf. Both the fractured and un-fractured contralateral femur specimens were evaluated. Polar moment of inertia (pMOI), tissue mineral density (TMD), bone volume fraction (BV/TV) were determined from microCT images, and callus diameter was determined from plain radiographs. Statistically significant differences in callus morphology as assessed by microCT were found between PAR-2(-/-) and PAR-2(+/+) mice at both 7 and 10 wpf. However, no significant histologic, plain radiographic, or biomechanical differences were found between the genotypes. The loss of PAR-2 was found to alter callus morphology as assessed by microCT but was not found to otherwise effect fracture healing in young mice. |
