| First Author | Huang X | Year | 2021 |
| Journal | Immunity | Volume | 54 |
| Issue | 7 | Pages | 1417-1432.e7 |
| PubMed ID | 34004142 | Mgi Jnum | J:316113 |
| Mgi Id | MGI:6740303 | Doi | 10.1016/j.immuni.2021.04.015 |
| Citation | Huang X, et al. (2021) Differential usage of transcriptional repressor Zeb2 enhancers distinguishes adult and embryonic hematopoiesis. Immunity 54(7):1417-1432.e7 |
| abstractText | The transcriptional repressor ZEB2 regulates development of many cell fates among somatic, neural, and hematopoietic lineages, but the basis for its requirement in these diverse lineages is unclear. Here, we identified a 400-basepair (bp) region located 165 kilobases (kb) upstream of the Zeb2 transcriptional start site (TSS) that binds the E proteins at several E-box motifs and was active in hematopoietic lineages. Germline deletion of this 400-bp region (Zeb2(Delta-165)mice) specifically prevented Zeb2 expression in hematopoietic stem cell (HSC)-derived lineages. Zeb2(Delta-165) mice lacked development of plasmacytoid dendritic cells (pDCs), monocytes, and B cells. All macrophages in Zeb2(Delta-165) mice were exclusively of embryonic origin. Using single-cell chromatin profiling, we identified a second Zeb2 enhancer located at +164-kb that was selectively active in embryonically derived lineages, but not HSC-derived ones. Thus, Zeb2 expression in adult, but not embryonic, hematopoiesis is selectively controlled by the -165-kb Zeb2 enhancer. |
