| First Author | Shang E | Year | 2009 |
| Journal | Dev Dyn | Volume | 238 |
| Issue | 4 | Pages | 908-17 |
| PubMed ID | 19301389 | Mgi Jnum | J:147040 |
| Mgi Id | MGI:3839160 | Doi | 10.1002/dvdy.21911 |
| Citation | Shang E, et al. (2009) Double bromodomain-containing gene Brd2 is essential for embryonic development in mouse. Dev Dyn 238(4):908-17 |
| abstractText | The BET subfamily of bromodomain-containing genes is characterized by the presence of two bromodomains and a unique ET domain at their carboxyl termini. Here, we show that the founding member of this subfamily, Brd2, is an essential gene by generating a mutant mouse line lacking Brd2 function. Homozygous Brd2 mutants are embryonic lethal, with most Brd2(-/-) embryos dying by embryonic day 11.5. Before death, the homozygous embryos were notably smaller and exhibited abnormalities in the neural tube where the gene is highly expressed. Brd2-deficient embryonic fibroblast cells were observed to proliferate more slowly than controls. Experiments to explore whether placental insufficiency could be a cause of the embryonic lethality showed that injecting diploid mutant embryonic stem cells into tetraploid wild-type blastocysts did not rescue the lethality; that is Brd2-deficient embryos could not be rescued by wild-type extraembryonic tissues. Furthermore, there were enhanced levels of cell death in Brd2-deficient embryos. Developmental Dynamics 238:908-917, 2009. (c) 2009 Wiley-Liss, Inc. |
