Primary Identifier | MGI:2152946 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Col5a2 |
Transmission | Germline | Strain of Origin | 129S4/SvJae |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A neomycin resistance cassette replaced a genomic fragment containing exon 6. RT-PCR analysis revelaled that homozygous mice produce in-frame transcripts from this allele lacking exon 6 sequence. Western blot analysis identified a protein produced from this allele that presumably lacks the N-telopeptide segment of the alpha2(V) chain. |