| Primary Identifier | MGI:2445400 | Allele Type | Targeted |
| Attribute String | Modified regulatory region | Gene | Rr349 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A 1312 bp sequence containing the Hba erythroid regulatory element (HS -26), located in Nprl3 intron 4 and containing transcription factor binding elements for the hemoglobin alpha genes, was replaced with a loxP site flanked neomycin resistance gene cassette. The neo cassette was removed through subsequent cre-mediated recombination, after which Nprl3 expression was restored to normal. The open reading frame of Nprl3 remained undisrupted. |
