| Primary Identifier | MGI:2445311 | Allele Type | Targeted |
| Attribute String | Inducible, Recombinase | Gene | Gt(ROSA)26Sor |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Induced With | tamoxifen | Is Recombinase | true |
| Is Wild Type | false |
| molecularNote | The endogenous locus was modified by insertion of a construct containing the rabbit beta-globin 3' splice site followed by the cre/ERT fusion gene, which encodes Cre recombinase joined to the G321R mutant hormone-binding domain of the human estrogen receptor that is activated by 4-hydroxytamoxifen (4-OHT), but not by the natural ER ligand 17-beta-estradiol. The fusion gene is followed by the rabbit beta-globin polyadenylation signal and a PGK-puro (puromycin resistance) cassette. Efficient Cre-mediated recombination in transgenic mice carrying floxed reporter genes was reported to reflect constitutive expression of the Gt(ROSA)26Sor from preimplantation stages onward. |
