| Primary Identifier | MGI:2445441 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(GFAP-Hras1)1Agu |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| description | This transgenic is designated RasD7 and by Southern blot analysis was found to contain multiple transgene insertions at multiple sites. All animals from this line died between 10-14 days of age. 50% of mice of a second line, Tg(GFAP-Hras1)2Agu, died by 4 weeks of age. |
| molecularNote | The transgene consists of the human GFAP promoter fused to an Hras1 cDNA, an IRES-lacZ cassette, and a floxed PGK-neo cassette. The human GFAP promoter directs transgene expression in the astrocytes.The Hras1 sequence contains an HA tag at the amino terminus. Western blot analysis of astrocytes from transgenic animals confirmed cell-specific expression of the transgene. |
