| Primary Identifier | MGI:2445446 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(GFAP-SV40T)1Tvd |
| Strain of Origin | (C57BL/6 x DBA/2)F1 | Is Recombinase | false |
| Is Wild Type | false |
| description | 10 out of 13 founder animals developed severe brain abnormalities and died by 7-9 days of age. This transgene differs from Tg(GFAP-TAg121)1Tvd, which is a derivative of Tg(GFAP-lacZ-TAg121)92Tvd, and contains a loxP site in place of a Cre deleted lacZ cassette. |
| molecularNote | The transgene consists of a 2.2 kb fragment of human GFAP promoter, active in central nervous system cells of the astrocyte lineage, and a mutant SV40 T antigen sequence, which contains a deletion for the small t antigen-specific splice signal and a deletion resulting in truncation of the large T antigen at amino acid 121. Transgene expression in central nervous system cells of the astrocyte lineage was observed by immunohistochemical staining of brain sections from transgenic animals. |
