| First Author | Djeghloul D | Year | 2016 |
| Journal | Stem Cell Reports | Volume | 6 |
| Issue | 6 | Pages | 970-984 |
| PubMed ID | 27304919 | Mgi Jnum | J:357956 |
| Mgi Id | MGI:6856132 | Doi | 10.1016/j.stemcr.2016.05.007 |
| Citation | Djeghloul D, et al. (2016) Age-Associated Decrease of the Histone Methyltransferase SUV39H1 in HSC Perturbs Heterochromatin and B Lymphoid Differentiation. Stem Cell Reports 6(6):970-984 |
| abstractText | The capacity of hematopoietic stem cells (HSC) to generate B lymphocytes declines with age, contributing to impaired immune function in the elderly. Here we show that the histone methyltransferase SUV39H1 plays an important role in human B lymphoid differentiation and that expression of SUV39H1 decreases with age in both human and mouse HSC, leading to a global reduction in H3K9 trimethylation and perturbed heterochromatin function. Further, we demonstrate that SUV39H1 is a target of microRNA miR-125b, a known regulator of HSC function, and that expression of miR-125b increases with age in human HSC. Overexpression of miR-125b and inhibition of SUV39H1 in young HSC induced loss of B cell potential. Conversely, both inhibition of miR-125 and enforced expression of SUV39H1 improved the capacity of HSC from elderly individuals to generate B cells. Our findings highlight the importance of heterochromatin regulation in HSC aging and B lymphopoiesis. |
