| First Author | Arthur-Farraj PJ | Year | 2017 |
| Journal | Cell Rep | Volume | 20 |
| Issue | 11 | Pages | 2719-2734 |
| PubMed ID | 28903050 | Mgi Jnum | J:254019 |
| Mgi Id | MGI:6103923 | Doi | 10.1016/j.celrep.2017.08.064 |
| Citation | Arthur-Farraj PJ, et al. (2017) Changes in the Coding and Non-coding Transcriptome and DNA Methylome that Define the Schwann Cell Repair Phenotype after Nerve Injury. Cell Rep 20(11):2719-2734 |
| abstractText | Repair Schwann cells play a critical role in orchestrating nerve repair after injury, but the cellular and molecular processes that generate them are poorly understood. Here, we perform a combined whole-genome, coding and non-coding RNA and CpG methylation study following nerve injury. We show that genes involved in the epithelial-mesenchymal transition are enriched in repair cells, and we identify several long non-coding RNAs in Schwann cells. We demonstrate that the AP-1 transcription factor C-JUN regulates the expression of certain micro RNAs in repair Schwann cells, in particular miR-21 and miR-34. Surprisingly, unlike during development, changes in CpG methylation are limited in injury, restricted to specific locations, such as enhancer regions of Schwann cell-specific genes (e.g., Nedd4l), and close to local enrichment of AP-1 motifs. These genetic and epigenomic changes broaden our mechanistic understanding of the formation of repair Schwann cell during peripheral nervous system tissue repair. |
