| Primary Identifier | MGI:2445839 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Icos |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Most of exon 2 and all of exons 3 and 4 were replaced by a neomycin selection cassette inserted by homologous recombination. The deleted region encoded the putative Icosl binding site, the transmembrane domain, and a portion of the intracellular domain including the YMFM motif that potentially binds lipid phosphotidylinositol 3 kinase. Flow cytometric analysis indicated an absence of protein on the surface of activated T cells obtained from homozygous mutant mice. |
