| First Author | Tian Y | Year | 2011 |
| Journal | Development | Volume | 138 |
| Issue | 7 | Pages | 1235-45 |
| PubMed ID | 21350014 | Mgi Jnum | J:171513 |
| Mgi Id | MGI:4950308 | Doi | 10.1242/dev.061762 |
| Citation | Tian Y, et al. (2011) Regulation of lung endoderm progenitor cell behavior by miR302/367. Development 138(7):1235-45 |
| abstractText | The temporal and spatial control of organ-specific endoderm progenitor development is poorly understood. miRNAs affect cell function by regulating programmatic changes in protein expression levels. We show that the miR302/367 cluster is a target of the transcription factor Gata6 in mouse lung endoderm and regulates multiple aspects of early lung endoderm progenitor development. miR302/367 is expressed at early stages of lung development, but its levels decline rapidly as development proceeds. Gain- and loss-of-function studies show that altering miR302/367 expression disrupts the balance of lung endoderm progenitor proliferation and differentiation, as well as apical-basal polarity. Increased miR302/367 expression results in the formation of an undifferentiated multi-layered lung endoderm, whereas loss of miR302/367 activity results in decreased proliferation and enhanced lung endoderm differentiation. miR302/367 coordinates the balance between proliferation and differentiation, in part, through direct regulation of Rbl2 and Cdkn1a, whereas apical-basal polarity is controlled by regulation of Tiam1 and Lis1. Thus, miR302/367 directs lung endoderm development by coordinating multiple aspects of progenitor cell behavior, including proliferation, differentiation and apical-basal polarity. |
