| First Author | Kananen K | Year | 1996 |
| Journal | Mol Cell Endocrinol | Volume | 119 |
| Issue | 2 | Pages | 135-46 |
| PubMed ID | 8807633 | Mgi Jnum | J:33603 |
| Mgi Id | MGI:81082 | Doi | 10.1016/0303-7207(96)03802-6 |
| Citation | Kananen K, et al. (1996) The mouse inhibin alpha-subunit promoter directs SV40 T-antigen to Leydig cells in transgenic mice [published erratum appears in Mol Cell Endocrinol 1996 Aug 30;122(1):109-10]. Mol Cell Endocrinol 119(2):135-46 |
| abstractText | Testicular tumorigenesis was observed in transgenic mice expressing the 6-kb mouse inhibin alpha-subunit promoter/Simian virus 40 T-antigen (SV40 Tag) fusion gene. The tumors were confined to Leydig cells using immunohistochemistry with anti-Tag antibody, specific binding of biotinylated hCG and histochemistry for 3 beta-hydroxysteroid dehydrogenase. Leydig cell hyperplasia and presence of Tag protein in the testicular interstitial tissue were already evident at 5 and 6.5 days of age, respectively. An immortalized cell line, BLT-1, was established from one testicular tumor. These cells expressed the LH receptor and P450scc mRNAs, and displayed LH-responsive cAMP and progesterone production, and low testosterone production. The cells also specifically bound 125I-labeled recombinant human LH with high affinity (36000 binding sites/cell), and the binding was regulated by 8Br-cAMP and hCG. This gonadal tumor model is valuable for further studies on endocrine functions of Leydig cells and their tumorigenesis in vivo and in vitro. |
