| Primary Identifier | MGI:2446342 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(Prrx1-Hand2)5Eno |
| Strain of Origin | (C57BL/6 x CBA)F1 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The transgene consists of a mouse Prrx1 promoter fused to a mutant Hand2 cDNA with the following amino acid subsitutions: arginine to glutamic acid at residues 109 (R109E) and 111 (R111E), and arginine to aspartic acid at residue 110 (R110D). The mouse Prrx1 promoter was used to drive transgene expression in the forelimb bud and anterior mesoderm of the hindlimb in E10.5 embryos. The Hand2 construct also contains an N-terminal Myc epitope tag and a 3' human growth hormone polyadenylation signal. |
