First Author | Wong HY | Year | 2021 |
Journal | STAR Protoc | Volume | 2 |
Issue | 1 | Pages | 100329 |
PubMed ID | 33644769 | Mgi Jnum | J:351427 |
Mgi Id | MGI:6728276 | Doi | 10.1016/j.xpro.2021.100329 |
Citation | Wong HY, et al. (2021) Whole-mount staining coupled to a UV-inducible basal cell carcinoma murine model. STAR Protoc 2(1):100329 |
abstractText | The origin of basal cell carcinomas (BCC) remains elusive. This protocol combines the use of an ultraviolet (UV)-inducible BCC murine model (K14CrexPtch(wt/lox)) and an optimized protocol for florescent whole-mount cytokeratin 17 immunostaining to address the spatiotemporal dynamics of BCC formation. The high-resolution three-dimensional confocal images are an excellent tool to visualize and quantify the distribution of skin cancers at a given time point. For complete details on the use and execution of this protocol, please refer to Roy et al. (2020). |