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Publication : Whole-mount staining coupled to a UV-inducible basal cell carcinoma murine model.

First Author  Wong HY Year  2021
Journal  STAR Protoc Volume  2
Issue  1 Pages  100329
PubMed ID  33644769 Mgi Jnum  J:351427
Mgi Id  MGI:6728276 Doi  10.1016/j.xpro.2021.100329
Citation  Wong HY, et al. (2021) Whole-mount staining coupled to a UV-inducible basal cell carcinoma murine model. STAR Protoc 2(1):100329
abstractText  The origin of basal cell carcinomas (BCC) remains elusive. This protocol combines the use of an ultraviolet (UV)-inducible BCC murine model (K14CrexPtch(wt/lox)) and an optimized protocol for florescent whole-mount cytokeratin 17 immunostaining to address the spatiotemporal dynamics of BCC formation. The high-resolution three-dimensional confocal images are an excellent tool to visualize and quantify the distribution of skin cancers at a given time point. For complete details on the use and execution of this protocol, please refer to Roy et al. (2020).
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