| First Author | Saito M | Year | 2020 |
| Journal | Cancer Sci | Volume | 111 |
| Issue | 8 | Pages | 2850-2860 |
| PubMed ID | 32535988 | Mgi Jnum | J:299389 |
| Mgi Id | MGI:6490856 | Doi | 10.1111/cas.14533 |
| Citation | Saito M, et al. (2020) CENP-50 is required for papilloma development in the two-stage skin carcinogenesis model. Cancer Sci 111(8):2850-2860 |
| abstractText | CENP-50/U is a component of the CENP-O complex (CENP-O/P/Q/R/U) and localizes to the centromere throughout the cell cycle. Aberrant expression of CENP-50/U has been reported in many types of cancers. However, as Cenp-50/U-deficient mice die during early embryogenesis, its functions remain poorly understood in vivo. To investigate the role of Cenp-50/U in skin carcinogenesis, we generated Cenp-50/U conditional knockout (K14Cre(ER) -Cenp-50/U(fl/fl) ) mice and subjected them to the 7,12-dimethylbenz(a)anthracene (DMBA)/terephthalic acid (TPA) chemical carcinogenesis protocol. As a result, early-stage papillomas decreased in Cenp-50/U-deficient mice. In contrast, Cenp-50/U-deficient mice demonstrated almost the same carcinoma incidence as control mice. Furthermore, mRNA expression analysis using DMBA/TPA-induced papillomas and carcinomas revealed that Cenp-50/U expression levels in papillomas were significantly higher than in carcinomas. These results suggest that Cenp-50/U functions mainly in early papilloma development and it has little effect on malignant conversion. |
