| First Author | Sugioka S | Year | 2022 |
| Journal | Biochem Biophys Res Commun | Volume | 614 |
| Pages | 169-174 | PubMed ID | 35597154 |
| Mgi Jnum | J:325727 | Mgi Id | MGI:7287236 |
| Doi | 10.1016/j.bbrc.2022.05.019 | Citation | Sugioka S, et al. (2022) Effects of constitutively active IKKbeta on cardiac development. Biochem Biophys Res Commun 614:169-174 |
| abstractText | NF-kappaB is a major transcription factor regulating cell survival, organ development and inflammation, but its role in cardiac development has been inadequately explored. To examine this function, we generated mice in which IKKbeta, an essential kinase for NF-kappaB activation, was constitutively activated in embryonic cardiomyocytes. For this purpose, we used smooth muscle-22alpha (SM22alpha)-Cre mice, which are frequently used for gene recombination in embryonic cardiomyocytes. Embryonic hearts of SM22alphaCre-CA (constitutively active) IKKbeta(flox/flox) mice revealed remarkably thin, spongy and hypoplastic myocardium. In exploring the mechanism, we found that the expression of bone morphogenetic protein 10 (BMP10) and T-box transcription factor 20 (Tbx20), major regulators of cardiac development, was significantly downregulated and upregulated, respectively, in the SM22alphaCre-CAIKKbeta(flox/flox) mice. We also generated NK2 homeobox 5 (Nkx2.5) Cre-CAIKKbeta(flox/wt) mice since Nkx2.5 is also expressed in embryonic cardiomyocytes and confirmed that the changes in these genes were also observed. These results implicated that the activation of NF-kappaB affects cardiac development. |
