| Primary Identifier | MGI:2446414 | Allele Type | Transgenic |
| Attribute String | Dominant negative, Inserted expressed sequence | Gene | Tg(CD2-Nfat5)1Snh |
| Strain of Origin | (BALB/c x C57BL/6)F1 | Is Recombinase | false |
| Is Wild Type | false |
| description | Two high-expressing transgenic founders, CD2DBD2 and CD2DBD10, were created and used for analysis. |
| molecularNote | A dominant negative form of mouse Nfat5 consisting of only the binding domain (amino acids 267-543) was cloned into a human CD2 expression vector and used as the transgene. The CD2 promoter is active in the thymus and mature peripheral T lymphocytes. Western blot analysis confirmed transgene expression in the thymus and spleen. |
