| First Author | van der Heijden ME | Year | 2021 |
| Journal | Elife | Volume | 10 |
| PubMed ID | 34542409 | Mgi Jnum | J:311546 |
| Mgi Id | MGI:6762324 | Doi | 10.7554/eLife.68045 |
| Citation | van der Heijden ME, et al. (2021) Maturation of Purkinje cell firing properties relies on neurogenesis of excitatory neurons. Elife 10:e68045 |
| abstractText | Preterm infants that suffer cerebellar insults often develop motor disorders and cognitive difficulty. Excitatory granule cells, the most numerous neuron type in the brain, are especially vulnerable and likely instigate disease by impairing the function of their targets, the Purkinje cells. Here, we use regional genetic manipulations and in vivo electrophysiology to test whether excitatory neurons establish the firing properties of Purkinje cells during postnatal mouse development. We generated mutant mice that lack the majority of excitatory cerebellar neurons and tracked the structural and functional consequences on Purkinje cells. We reveal that Purkinje cells fail to acquire their typical morphology and connectivity, and that the concomitant transformation of Purkinje cell firing activity does not occur either. We also show that our mutant pups have impaired motor behaviors and vocal skills. These data argue that excitatory cerebellar neurons define the maturation time-window for postnatal Purkinje cell functions and refine cerebellar-dependent behaviors. |
