| Primary Identifier | MGI:2446449 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(ACTA1-UCP3)1Clap |
| Strain of Origin | (C57BL/6 x CBA)F2 | Is Recombinase | false |
| Is Wild Type | false |
| description | Three transgenic lines were created and bred to homozygosity. The highest expressing line was used for characterization. Quantitative RT-PCR analysis showed that transgene expression is confined to skeletal muscle and brown adipose tissue. Transgene expression is 66-fold of wild-type in skeletal muscle and 50% of wild-type in brown adipose tissue. |
| molecularNote | The transgene consists of a human ACTA1 promoter and a human UCP3 gene. ACTA1 drives transgene expression in skeletal muscle. |
