| Primary Identifier | MGI:2446530 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(Abpa-FSHR*D567G)1Cmal |
| Strain of Origin | (C3H/HeH x 101/H)F1 | Is Recombinase | false |
| Is Wild Type | false |
| description | Fertilized oocytes derived from (C3H/HeH x 101/H)F1 animals carrying the Gnrh1hpg mutation were used for transgene injection. Two transgenic founder lines, RR3 and RR4, were established and used for analysis. |
| molecularNote | The transgene consists of a rat Abpa promoter and a human FSHR minigene. The FSHR minigene contains exon 1, a shortened intron 1 lacking the midregion, and exons 2-10 with no intervening introns. The minigene also includes a mutation causing an aspartic acid to glycine change at amino acid residue 567. The rat Abpa promoter directs transgene expression in Sertoli cells. Transgene expression was confirmed by RT-PCR analysis of testes and ovary total RNA; expression was also detected in the brain and, at lower levels, in the pituitary. |
