Primary Identifier | MGI:2447446 | Allele Type | Transgenic |
Attribute String | Inserted expressed sequence | Gene | Tg(Amh-Smad4)1Tkw |
Strain of Origin | CD-1 | Is Recombinase | false |
Is Wild Type | false |
description | Transgenic line B showed the highest level of transgene expression and was used for further study. |
molecularNote | The transgene consists of a Smad4 cDNA under the control of the anti-Mullerian hormone (Amh) promoter. A FLAG epitope tag and human growth hormone polyadenylation sequence was also added to the construct. Transgene expression was confirmed by Western blot analysis of testes extracts from neonatal transgenic mice. |