| Primary Identifier | MGI:2447593 | Allele Type | Transgenic |
| Attribute String | Humanized sequence, Inserted expressed sequence | Gene | Tg(LCKprBCL2L1)12Sjk |
| Strain of Origin | Not Specified | Is Recombinase | false |
| Is Wild Type | false |
| description | Transgenic lines 12 and 24, which expressed high amounts of the transgene, were established. Most of the analysis was performed using line 12 and confirmed with line 24. |
| molecularNote | The transgene consists of 0.75 kb of Lck proximal promoter sequences and 0.8 kb of human BCL2L1 cDNA. Introns, exons, and polyadenylation sequences from the human growth hormone gene consitutes the 3' untranslated region of the construct. The Lck promoter was used to drive transgene expression in lymphoid tissues. Transgene expression in the thymus and spleen was verified by Western blot analysis and flow cytometry. |
