| Primary Identifier | MGI:2447611 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Mbd4 |
| Transmission | Germline | Strain of Origin | STOCK 129/Sv and C57BL/6J and SJL |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The gene was disrupted by replacement of exon 3 and part of intron 3 with a PGK-neo cassette via homologous recombination. The targeted mutation results in deletion of the catalytic domain encoded by exon 3. RT-PCR analysis of RNA from homozygous mutant animals confirmed deletion of exon 3 and revealed a low level exon2-exon4 alternative splice product. The predicted product contains a frameshift mutation resulting in a nonfunctional 125 amino acid protein, of which 98 amino acids are Mbd4-derived. |
