| Primary Identifier | MGI:2447298 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Mid1 |
| Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<b-m2> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The gene was replaced by a puromycin resistance gene and a loxP site in ES cells in which Arhgap6 had already been subjected to targeted mutation to replace exons 6, 7, and 8 with a neomycin resistance cassette and a loxP site. The two loxP sites are situated so that cre excision eliminates both resistance genes and all intervening DNA. All of Mid1, the entire Hccs gene, and all of Arhgap6 3' of exon 5 are thus conditionally deleted. |
