| First Author | Johansson E | Year | 2010 |
| Journal | Biochem Biophys Res Commun | Volume | 396 |
| Issue | 2 | Pages | 407-12 |
| PubMed ID | 20417186 | Mgi Jnum | J:162518 |
| Mgi Id | MGI:4819081 | Doi | 10.1016/j.bbrc.2010.04.105 |
| Citation | Johansson E, et al. (2010) Glutathione deficient C57BL/6J mice are not sensitized to ozone-induced lung injury. Biochem Biophys Res Commun 396(2):407-12 |
| abstractText | In this study we examined the role of the antioxidant glutathione (GSH) in pulmonary susceptibility to ozone toxicity, utilizing GSH deficient C57BL/6J mice that lack the expression of glutamate-cysteine ligase modifier subunit (GCLM). Gclm(-/-) knockout mice had 70% GSH depletion in the lung. Gclm(+/+) wild-type and Gclm(-/-) mice were exposed to either 0.3 ppm ozone or filtered air for 48h. Ozone-induced lung hyperpermeability, as measured by total protein concentration in bronchoalveolar lavage fluid, was surprisingly lower in Gclm(-/-) mice than in wild-type mice. Lung hyperpermeability did not correlate with the degree of neutrophilia or with inflammatory gene expression. Pulmonary antioxidant response to ozone, assessed by increased mRNA levels of metallothionein 1 and 2, alpha-tocopherol transporter protein, and solute carrier family 23 member 2 (sodium-dependent vitamin C transporter) was greater in Gclm(-/-) mice than in Gclm(+/+) mice. These results suggest that compensatory augmentation of antioxidant defenses in Gclm(-/-) mice may confer increased resistance to ozone-induced lung injury. |
