| Primary Identifier | MGI:2447657 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(NOS3)2Crom |
| Strain of Origin | FVB | Is Recombinase | false |
| Is Wild Type | false |
| description | Two transgenic lines, eNOStg2 and eNOStg3, were created. Line eNOStg2 expresses the highest amount of human transgene product. In the aorta, eNOStg2 expresses 10-fold the amount of active enzyme compared to wild-type. |
| molecularNote | The entire human NOS3 genomic sequence and flanking regions was used for the transgene. Transgene expression was confirmed by Western blot analysis of aorta homogenates. Transgene expression was also observed by immunohistochemistry. Blood vessels and capillaries of heart sections from transgenic animals stained positive for human NOS3, as did sinusoids of the liver and kidney and capillaries of the glomeruli. |
