| First Author | Perry RB | Year | 2016 |
| Journal | Cell Rep | Volume | 16 |
| Issue | 6 | Pages | 1664-1676 |
| PubMed ID | 27477284 | Mgi Jnum | J:238977 |
| Mgi Id | MGI:5824645 | Doi | 10.1016/j.celrep.2016.07.005 |
| Citation | Perry RB, et al. (2016) Nucleolin-Mediated RNA Localization Regulates Neuron Growth and Cycling Cell Size. Cell Rep 16(6):1664-76 |
| abstractText | How can cells sense their own size to coordinate biosynthesis and metabolism with their growth needs? We recently proposed a motor-dependent bidirectional transport mechanism for axon length and cell size sensing, but the nature of the motor-transported size signals remained elusive. Here, we show that motor-dependent mRNA localization regulates neuronal growth and cycling cell size. We found that the RNA-binding protein nucleolin is associated with importin beta1 mRNA in axons. Perturbation of nucleolin association with kinesins reduces its levels in axons, with a concomitant reduction in axonal importin beta1 mRNA and protein levels. Strikingly, subcellular sequestration of nucleolin or importin beta1 enhances axonal growth and causes a subcellular shift in protein synthesis. Similar findings were obtained in fibroblasts. Thus, subcellular mRNA localization regulates size and growth in both neurons and cycling cells. |
