| First Author | Hande S | Year | 1998 |
| Journal | Immunity | Volume | 8 |
| Issue | 2 | Pages | 189-98 |
| PubMed ID | 9492000 | Mgi Jnum | J:88411 |
| Mgi Id | MGI:3032967 | Doi | 10.1016/s1074-7613(00)80471-9 |
| Citation | Hande S, et al. (1998) Bcl-2 obstructs negative selection of autoreactive, hypermutated antibody V regions during memory B cell development. Immunity 8(2):189-98 |
| abstractText | We analyzed the participation of a predominant B cell clonotype in the anti-arsonate immune response of mice in which Bcl-2 expression was enforced in B cells. Many of the antibodies expressed by the arsonate-induced memory compartment of these mice were 'dual-reactive,' displaying increased affinity acquired via V region somatic hypermutation for both arsonate and the autoantigen DNA. The hypermutated antibodies expressed by the anti-arsonate memory B cell compartment of normal mice have increased affinity for arsonate but lack measurable affinity for DNA. Thus, interference with apoptotic pathways allows developing memory B cells that have acquired autoreactivity to bypass a peripheral tolerance checkpoint. These data demonstrate that both positive and negative selection, working in concert with V gene somatic hypermutation, result in the 'specificity maturation' of the antibody response. |
