| First Author | Bartlett JD | Year | 2006 |
| Journal | J Dent Res | Volume | 85 |
| Issue | 10 | Pages | 894-9 |
| PubMed ID | 16998127 | Mgi Jnum | J:267865 |
| Mgi Id | MGI:6259206 | Doi | 10.1177/154405910608501004 |
| Citation | Bartlett JD, et al. (2006) Origin, splicing, and expression of rodent amelogenin exon 8. J Dent Res 85(10):894-9 |
| abstractText | Amelogenin RNA transcripts undergo extensive alternative splicing, and MMP-20 processes the isoforms following their secretion. Since amelogenins have been ascribed cell-signaling activities, we asked if a lack of proteolytic processing by MMP-20 affects amelogenin signaling and consequently alters amelogenin splice site selection. RT-PCR analyses of amelogenin mRNA between control and Mmp20(-/-)mice revealed no differences in the splicing pattern. We characterized 3 previously unidentified amelogenin alternatively spliced transcripts and demonstrated that exon-8-encoded amelogenin isoforms are processed by MMP-20. Transcripts with exon 8 were expressed approximately five-fold less than those with exon 7. Analyses of the mouse and rat amelogenin gene structures confirmed that exon 8 arose in a duplication of exons 4 through 5, with translocation of the copy downstream of exon 7. No downstream genomic sequences homologous to exons 4-5 were present in the bovine or human amelogenin genes, suggesting that this translocation occurred only in rodents. |
