| First Author | Stoppelkamp S | Year | 2015 |
| Journal | Mol Immunol | Volume | 67 |
| Issue | 2 Pt B | Pages | 398-406 |
| PubMed ID | 26216045 | Mgi Jnum | J:233110 |
| Mgi Id | MGI:5780790 | Doi | 10.1016/j.molimm.2015.07.002 |
| Citation | Stoppelkamp S, et al. (2015) Murine pattern recognition receptor dectin-1 is essential in the development of experimental autoimmune uveoretinitis. Mol Immunol 67(2 Pt B):398-406 |
| abstractText | Mycobacteria in complete Freund's adjuvant (CFA) are an essential component of immunization protocols in a number of autoimmune disease animal models including experimental autoimmune encephalomyelitis and uveoretinitis (EAE and EAU, respectively). We determined the role in EAU of two C-type lectin receptors on myeloid cells that recognize and respond to mycobacteria. Using receptor-specific antibodies and knockout mice, we demonstrated for the first time that the macrophage mannose receptor delays disease development but does not affect severity. In contrast, dectin-1 is critically involved in the development of CFA-mediated EAU. Disease severity is reduced in dectin-1 knockout mice and antibody blockade of dectin-1 during the induction, but not the effector phase, prevents EAU development. Significantly, similar blockade of dectin-1 in vivo has no effect in non-CFA-mediated, spontaneously induced or adoptive transfer models of EAU. Thus dectin-1 plays a critical role in the ability of complete Freund's adjuvant to induce EAU in mice. |
