| First Author | Jansen KM | Year | 2006 |
| Journal | J Cell Biol | Volume | 174 |
| Issue | 3 | Pages | 403-13 |
| PubMed ID | 16864654 | Mgi Jnum | J:111306 |
| Mgi Id | MGI:3653577 | Doi | 10.1083/jcb.200601102 |
| Citation | Jansen KM, et al. (2006) Mannose receptor regulates myoblast motility and muscle growth. J Cell Biol 174(3):403-13 |
| abstractText | Myoblast fusion is critical for the formation, growth, and maintenance of skeletal muscle. The initial formation of nascent myotubes requires myoblast-myoblast fusion, but further growth involves myoblast-myotube fusion. We demonstrate that the mannose receptor (MR), a type I transmembrane protein, is required for myoblast-myotube fusion. Mannose receptor (MR)-null myotubes were small in size and contained a decreased myonuclear number both in vitro and in vivo. We hypothesized that this defect may arise from a possible role of MR in cell migration. Time-lapse microscopy revealed that MR-null myoblasts migrated with decreased velocity during myotube growth and were unable to migrate in a directed manner up a chemoattractant gradient. Furthermore, collagen uptake was impaired in MR-null myoblasts, suggesting a role in extracellular matrix remodeling during cell motility. These data identify a novel function for MR during skeletal muscle growth and suggest that myoblast motility may be a key aspect of regulating myotube growth. |
