| First Author | Song P | Year | 2011 |
| Journal | Circ Res | Volume | 109 |
| Issue | 11 | Pages | 1230-9 |
| PubMed ID | 21980125 | Mgi Jnum | J:196559 |
| Mgi Id | MGI:5488721 | Doi | 10.1161/CIRCRESAHA.111.250423 |
| Citation | Song P, et al. (2011) AMPKalpha2 deletion exacerbates neointima formation by upregulating Skp2 in vascular smooth muscle cells. Circ Res 109(11):1230-9 |
| abstractText | RATIONALE: Adenosine monophosphate-activated protein kinase (AMPK), a metabolic and redox sensor, is reported to suppress cell proliferation of nonmalignant and tumor cells. Whether AMPKalpha alters vascular neointima formation induced by vascular injury is unknown. OBJECTIVE: The aim of this study was to determine the roles of AMPKalpha in the development of vascular neointima hyperplasia and to elucidate the underlying mechanisms. METHODS AND RESULTS: Vascular smooth muscle cell (VSMC) proliferation and neointimal hyperplasia were evaluated in cultured VSMCs and wire-injured mouse carotid arteries from wild-type (WT, C57BL/6J), AMPKalpha2(-/-), and AMPKalpha1(-/-) mice. Mouse VSMCs derived from aortas of AMPKalpha2(-/-) mice exhibited increased proliferation compared with either WT or AMPKalpha1(-/-) VSMCs. Further, deletion of AMPKalpha2 but not AMPKalpha1 reduced the level of p27(Kip1), a cyclin-dependent kinase inhibitor, and increased the level of S-phase kinase-associated protein 2 (Skp2), a known E3 ubiquitin ligase for p27(Kip1), through activation of p52 nuclear factor kappa B (NF-kappaB)-2. Moreover, either pharmacological (ie, through compound C) or genetical (ie, through AMPKalpha2-specific siRNA) inhibition of AMPK decreased p27(Kip1) levels but increased the abundance of Skp2 in human VSMCs. Furthermore, gene silencing of Skp2 reversed the levels of p27(Kip1) and VSMCs proliferation. Finally, neointima formation after mechanical arterial injury was increased in AMPKalpha2(-/-) but not AMPKalpha1(-/-) mice. CONCLUSIONS: These findings indicate that deletion of AMPKalpha2 through p52-Skp2-mediated ubiquitination and degradation of p27(Kip1) accentuates neointimal hyperplasia in response to wire injury. |
