| First Author | Chen WT | Year | 2005 |
| Journal | Mol Imaging | Volume | 4 |
| Issue | 1 | Pages | 67-74 |
| PubMed ID | 15967128 | Mgi Jnum | J:104334 |
| Mgi Id | MGI:3611677 | Doi | 10.1162/15353500200504199 |
| Citation | Chen WT, et al. (2005) Detection of dysplastic intestinal adenomas using a fluorescent folate imaging probe. Mol Imaging 4(1):67-74 |
| abstractText | Macrophages have long been recognized as a prominent component of tumors. Activated macrophages overexpress folate receptors and we used this phenomenon to image inflammatory reactions in colon dysplasia using a fluorescent folate probe (FFP). APC(Delta468) mice injected with FFP showed fluorescent adenomas (target-to-background ratio, adenoma vs. adjacent normal mucosa, of 2.46 +/- 0.41), significantly higher (p < .001) than adenomas in animals injected with a non-folate-containing control probe. Fluorescence-activated cell-sorting analysis revealed a 3-fold higher content of Mac1-positive cells in colonic adenomas compared with normal adjacent mucosa (6.8% vs. 2.2%), and confirmed the source of FFP-positive cells to be primarily an F4/80-positive macrophage subpopulation. Taken together, these results indicate that probe potentially can be used to image dysplastic intestinal adenomas in vivo. |
