| First Author | Almonacid M | Year | 2019 |
| Journal | Dev Cell | Volume | 51 |
| Issue | 2 | Pages | 145-157.e10 |
| PubMed ID | 31607652 | Mgi Jnum | J:284138 |
| Mgi Id | MGI:6389707 | Doi | 10.1016/j.devcel.2019.09.010 |
| Citation | Almonacid M, et al. (2019) Active Fluctuations of the Nuclear Envelope Shape the Transcriptional Dynamics in Oocytes. Dev Cell 51(2):145-157.e10 |
| abstractText | Nucleus position in cells can act as a developmental cue. Mammalian oocytes position their nucleus centrally using an F-actin-mediated pressure gradient. The biological significance of nucleus centering in mammalian oocytes being unknown, we sought to assess the F-actin pressure gradient effect on the nucleus. We addressed this using a dedicated computational 3D imaging approach, biophysical analyses, and a nucleus repositioning assay in mouse oocytes mutant for cytoplasmic F-actin. We found that the cytoplasmic activity, in charge of nucleus centering, shaped the nucleus while promoting nuclear envelope fluctuations and chromatin motion. Off-centered nuclei in F-actin mutant oocytes were misshaped with immobile chromatin and modulated gene expression. Restoration of F-actin in mutant oocytes rescued nucleus architecture fully and gene expression partially. Thus, the F-actin-mediated pressure gradient also modulates nucleus dynamics in oocytes. Moreover, this study supports a mechano-transduction model whereby cytoplasmic microfilaments could modulate oocyte transcriptome, essential for subsequent embryo development. |
