| First Author | Li SK | Year | 2015 |
| Journal | J Immunol | Volume | 194 |
| Issue | 8 | Pages | 3798-807 |
| PubMed ID | 25769919 | Mgi Jnum | J:308371 |
| Mgi Id | MGI:6728885 | Doi | 10.4049/jimmunol.1402432 |
| Citation | Li SK, et al. (2015) Identification of a negative regulatory role for spi-C in the murine B cell lineage. J Immunol 194(8):3798-807 |
| abstractText | Spi-C is an E26 transformation-specific family transcription factor that is highly related to PU.1 and Spi-B. Spi-C is expressed in developing B cells, but its function in B cell development and function is not well characterized. To determine whether Spi-C functions as a negative regulator of Spi-B (encoded by Spib), mice were generated that were germline knockout for Spib and heterozygous for Spic (Spib(-/-)Spic(+/-)). Interestingly, loss of one Spic allele substantially rescued B cell frequencies and absolute numbers in Spib(-/-) mouse spleens. Spib(-/-)Spic(+/-) B cells had restored proliferation compared with Spib(-/-) B cells in response to anti-IgM or LPS stimulation. Investigation of a potential mechanism for the Spib(-/-)Spic(+/-) phenotype revealed that steady-state levels of Nfkb1, encoding p50, were elevated in Spib(-/-)Spic(+/-) B cells compared with Spib(-/-) B cells. Spi-B was shown to directly activate the Nfkb1 gene, whereas Spi-C was shown to repress this gene. These results indicate a novel role for Spi-C as a negative regulator of B cell development and function. |
