| Primary Identifier | MGI:2448738 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(BCL2/IGH)1Tjd |
| Strain of Origin | (C57BL/6 x C3H/He)F1 | Is Recombinase | false |
| Is Wild Type | false |
| description | Five transgenic lines (M-14, M-30, M-43, M-59, and M-62) were established. |
| molecularNote | A human genomic BCL2/IGH fusion minigene was used for the transgene. This construct represents a translocation mutation, t(14:18), found in human follicular B cell lymphomas. The entire wild-type sequence of BCL2 was used. Because of the large size of BCL2 intron 2 (350 kb), a cDNA segment of the exon II/exon III junction was substituted. Transgene expression in the spleen and thymus was confirmed by S1 nuclease protection assay. |
