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Publication : Constitutive turnover of cyclin E by Cul3 maintains quiescence.

First Author  McEvoy JD Year  2007
Journal  Mol Cell Biol Volume  27
Issue  10 Pages  3651-66
PubMed ID  17339333 Mgi Jnum  J:122228
Mgi Id  MGI:3713609 Doi  10.1128/MCB.00720-06
Citation  McEvoy JD, et al. (2007) Constitutive turnover of cyclin E by Cul3 maintains quiescence. Mol Cell Biol 27(10):3651-66
abstractText  Two distinct pathways for the degradation of mammalian cyclin E have previously been described. One pathway is induced by cyclin E phosphorylation and is dependent on the Cul1/Fbw7-based E3 ligase. The other pathway is dependent on the Cul3-based E3 ligase, but the mechanistic details of this pathway have yet to be elucidated. To establish the role of Cul3 in the degradation of cyclin E in vivo, we created a conditional knockout of the Cul3 gene in mice. Interestingly, the biallelic loss of Cul3 in primary fibroblasts derived from these mice results in increased cyclin E expression and reduced cell viability, paralleling the loss of Cul3 protein expression. Cell cycle analysis of viable, Cul3 hypomorphic cells shows that decreasing the levels of Cul3 increases both cyclin E protein levels and the number of cells in S phase. In order to examine the role of Cul3 in an in vivo setting, we determined the effect of deletion of the Cul3 gene in liver. This gene deletion resulted in a dramatic increase in cyclin E levels as well as an increase in cell size and ploidy. The results we report here show that the constitutive degradation pathway for cyclin E that is regulated by the Cul3-based E3 ligase is essential to maintain quiescence in mammalian cells.
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