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Publication : An improved Flp deleter mouse in C57Bl/6 based on Flpo recombinase.

First Author  Kranz A Year  2010
Journal  Genesis Volume  48
Issue  8 Pages  512-20
PubMed ID  20506501 Mgi Jnum  J:160215
Mgi Id  MGI:4453889 Doi  10.1002/dvg.20641
Citation  Kranz A, et al. (2010) An improved Flp deleter mouse in C57Bl/6 based on Flpo recombinase. Genesis 48(8):512-20
abstractText  Recently, a codon improved version of the Flpe site specific recombinase, termed Flpo, was reported as having greatly improved performance in mammalian cell applications. However, the degree of improvement could not be estimated because essentially no Flpe activity was observed. Here, we compare Flpe and Flpo accurately in a mammalian cell assay to estimate that Flpo is about five times more active than Flpe and similar to Cre and Dre. Consequently, we generated a Flpo deleter mouse line from the JM8 C57Bl/6 ES cells used in the EUCOMM and KOMP systematic knock-out programs. In breeding experiments, we show that the Flpo deleter delivers complete recombination using alleles that are incompletely recombined by a commonly used Flpe deleter. This indicates that the Flpo deleter is more efficient.
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