| Primary Identifier | MGI:2450287 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Trp53 |
| Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<b-m2> |
| Is Recombinase | false | Is Wild Type | false |
| description | Oppositely-oriented loxP sites in Trp53tm2Brd and Wnt3tm2Brd, in cis, served as the proximal and distal breakpoints, respectively, of a 24-cM Cre recombinase-mediated inversion, In(11Trp53;11Wnt3)8Brd. (J:56548) Passage of the two targeted mutations through the germline without exposure to Cre recombinase resulted in their segregation to separate Chr 16 homologs and thus to their independent inheritance. (J:80303 ) |
| molecularNote | A 0.6-kb genomic DNA fragment containing exon 2, including the translation initiation codon, exon 3, and part of exon 4 was replaced with a cassette containing a puromycin resistance gene, a loxP site oriented distally (away from the centromere), the 3' half of an Hprt minigene, and a tyrosinase (Tyr) minigene in an ES cell line already harboring Wnt3tm2Brd. The cassette integrated into the same Chromosome 16 homolog, in cis with Wnt3tm2Brd. |
