| First Author | Nowakowski TJ | Year | 2013 |
| Journal | Dev Biol | Volume | 382 |
| Issue | 2 | Pages | 530-7 |
| PubMed ID | 24012747 | Mgi Jnum | J:210198 |
| Mgi Id | MGI:5569703 | Doi | 10.1016/j.ydbio.2013.08.023 |
| Citation | Nowakowski TJ, et al. (2013) Loss of functional Dicer in mouse radial glia cell-autonomously prolongs cortical neurogenesis. Dev Biol 382(2):530-7 |
| abstractText | Radial glia of the mouse cerebral cortex emerge from neuroepithelial stem cells around embryonic day 11 and produce excitatory cortical neurons until a few days before birth. The molecular mechanisms that regulate the end of cortical neurogenesis remain largely unknown. Here we investigated if the Dicer-dependent microRNA (miRNA) pathway is involved. By electroporating a cre-recombinase expression vector into the cortex of E13.5 embryos carrying a conditional allele of Dicer1, we induced mosaic recombination causing Dicer1 deletion and reporter activation in a subset of radial glia. We analysed the long-term fates of their progeny. We found that mutant radial glia produced abnormally large numbers of Cux1-positive neurons, many of which populated the superficial cortical layers. Injections of the S-phase marker bromodeoxyuridine between postnatal days 3 and 14 showed that much of this population was generated postnatally. Our findings suggest a role for Dicer-dependent processes in limiting the timespan of cortical neurogenesis. |
