| First Author | Zhan X | Year | 2015 |
| Journal | Genesis | Volume | 53 |
| Issue | 7 | Pages | 440-8 |
| PubMed ID | 26077106 | Mgi Jnum | J:224076 |
| Mgi Id | MGI:5661245 | Doi | 10.1002/dvg.22866 |
| Citation | Zhan X, et al. (2015) Generation of BAF53b-Cre transgenic mice with pan-neuronal Cre activities. Genesis 53(7):440-8 |
| abstractText | Molecular and functional studies of genes in neurons in mouse models require neuron-specific Cre lines. The current available neuronal Cre transgenic or knock-in lines either result in expression in a subset of neurons or expression in both neuronal and non-neuronal tissues. Previously we identified BAF53b as a neuron-specific subunit of the chromatin remodeling BAF complexes. Using a bacteria artificial chromosome (BAC) construct containing the BAF53b gene, we generated a Cre transgenic mouse under the control of BAF53b regulatory elements. Like the endogenous BAF53b gene, we showed that BAF53b-Cre is largely neuron-specific. In both central and peripheral nervous systems, it was expressed in all developing neurons examined and was not observed in neural progenitors or glial cells. In addition, BAF53b-Cre functioned in primary cultures in a pan-neuron-specific manner. Thus, BAF53b-Cre mice will be a useful genetic tool to manipulate gene expression in developing neurons for molecular, biochemical, and functional studies. genesis, 53:440-448, 2015. (c) 2015 Wiley Periodicals, Inc. |
