| First Author | Castrillon C | Year | 2023 |
| Journal | Elife | Volume | 12 |
| PubMed ID | 37341394 | Mgi Jnum | J:354073 |
| Mgi Id | MGI:7719118 | Doi | 10.7554/eLife.81012 |
| Citation | Castrillon C, et al. (2023) Complex subsets but redundant clonality after B cells egress from spontaneous germinal centers. Elife 12 |
| abstractText | Affinity matured self-reactive antibodies are found in autoimmune diseases like systemic lupus erythematous. Here, we used fate-mapping reporter mice and single-cell transcriptomics coupled to antibody repertoire analysis to characterize the post-germinal center (GC) B cell compartment in a new mouse model of autoimmunity. Antibody-secreting cells (ASCs) and memory B cells (MemBs) from spontaneous GCs grouped into multiple subclusters. ASCs matured into two terminal clusters, with distinct secretion, antibody repertoire and metabolic profiles. MemBs contained FCRL5+ and CD23+ subsets, with different in vivo localization in the spleen. GC-derived FCRL5+ MemBs share transcriptomic and repertoire properties with atypical B cells found in aging and infection and localize to the marginal zone, suggesting a similar contribution to recall responses. While transcriptomically diverse, ASC and MemB subsets maintained an underlying clonal redundancy. Therefore, self-reactive clones could escape subset-targeting therapy by perpetuation of self-reactivity in distinct subsets. |
