| Primary Identifier | MGI:2449704 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Prkg2 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A 308 bp fragment of the second coding exon was replaced with a neomycin selection cassette inserted by homologous recombination. The disrupted exon encoded a portion of the cGMP binding pocket. Northern blot, RT-PCR, and Western blot analyses indicated an absence of normal transcript and protein in homozygous mutant mice. Functional ablation was confirmed by a lack of cGMP-stimulated phosphotransferase activity in jejunal homogenates. |
